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Please use this identifier to cite or link to this item: http://ntour.ntou.edu.tw:8080/ir/handle/987654321/47368

Title: Evolution of Gold Nanoparticle Clusters in Living Cells Studied by Sectional Dark-Field Optical Microscopy and Chromatic Analysis
Authors: Pei-Kuen Wei
Sheng-Hann Wang
Chia-Wei Lee
Fan-Gang Tseng
Kuo-Kan Liang
Contributors: 國立臺灣海洋大學:光電科學研究所
Date: 2016-03
Issue Date: 2018-07-17T02:34:14Z
Publisher: J. Biophotonics
Abstract: Abstract: The evolution of gold nanoparticle (Au NP) clusters in living cells are studied by using sectional dark-field optical microscopy and chromatic analysis approach. During endocytosis, Au NP clusters undergo fantastic color changes, from green to yellow-orange due to the plasmonic coupling effect. Analysis of brightness/hue values of the dark-field images helps estimate the numbers of Au NPs in the clusters. The Au NP clusters were further categorized into four groups within the endocytosis. As the results, the late endosomes had increased number of large Au NP clusters with time, while clustered numbers in secondary and tertiary groups were first increased and then decreased due to the fusion and fission of the endocytic vesicles. The time constants and cluster numbers for different groups are fitted by using an integrated rate equation, and show a positive correlation with the size of the Au NP cluster. The efficiency of Au NP uptake is only about 50% for normal cells, while 75% for cancer cells. Compared to normal cells, cancer cells show a larger number in uptake, while faster rate in removal. The propose method helps the kinetic study of endocytosed nanoparticles in physiological conditions.
Relation: 9(7) pp.738-749
URI: http://ntour.ntou.edu.tw:8080/ir/handle/987654321/47368
Appears in Collections:[光電科學研究所] 期刊論文

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