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Please use this identifier to cite or link to this item: http://ntour.ntou.edu.tw:8080/ir/handle/987654321/47305

Title: Phase TEM for biological imaging utilizing a Boersch electrostatic phase plate: theory and practice
Authors: Chen FR
Hsu CH
Huang SH
Chang CS
Shiue J
Tsai JS
Chang WH
Wu YM
Lin YC
Kuo PC
Huang YS
Hwu Y
Kai JJ
Tseng FG
Contributors: 國立臺灣海洋大學:光電科學研究所
Date: 2009-06
Issue Date: 2018-07-16T01:42:56Z
Publisher: JOURNAL OF ELECTRON MICROSCOPY
Abstract: Abstract: A Boersch electrostatic phase plate (BEPP) used in a transmission electron microscope (TEM) system can provide tuneable phase shifts and overcome the low contrast problem for biological imaging. Theoretically, a pure phase image with a high phase contrast can be obtained using a BEPP. However, a currently available TEM system utilizing a BEPP cannot achieve sufficiently high phase efficiency for biological imaging, owing to the practical conditions. The low phase efficiency is a result of the blocking of partial unscattered electrons by BEPP, and the contribution of absorption contrast. The fraction of blocked unscattered beam is related to BEPP dimensions and to divergence of the illumination system of the TEM. These practical issues are discussed in this paper. Phase images of biological samples (negatively stained ferritin) obtained by utilizing a BEPP are reported, and the phase contrast was found to be enhanced by a factor of approximately 1.5, based on the calculation using the Rose contrast criterion. The low gain in phase contrast is consistent with the expectation from the current TEM/BEPP system. A new generation of phase TEM utilizing BEPP and designed for biological imaging with a high phase efficiency is proposed.
Relation: 58(3)
URI: http://ntour.ntou.edu.tw:8080/ir/handle/987654321/47305
Appears in Collections:[光電科學研究所] 期刊論文

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