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Please use this identifier to cite or link to this item: http://ntour.ntou.edu.tw:8080/ir/handle/987654321/47277

Title: Quantitative analysis of nanoparticle internalization in mammalian cells by high resolution X-ray microscopy
Authors: Giorgio Margaritondo
Hsiang-Hsin Chen
Chia-Chi Chien
Cyril Petibois
Cheng-Liang Wang
Yong S Chu
Sheng-Feng Lai
Tzu-En Hua
Yi-Yun Chen
Xiaoqing Cai
Ivan M Kempson
Yeukuang Hwu
Contributors: 國立臺灣海洋大學:光電科學研究所
Keywords: Nanoparticle Uptake
HeLa Cell
Tomographic Reconstruction
Nanoparticle Colloid
Cluster Size Distribution
Date: 2011-04
Issue Date: 2018-07-10T01:04:51Z
Publisher: JOURNAL OF NANOBIOTECHNOLOGY
Abstract: Abstract: Background:Quantitative analysis of nanoparticle uptake at the cellular level is critical to nanomedicine procedures. In particular, it is required for a realistic evaluation of their effects. Unfortunately, quantitative measurements of nanoparticle uptake still pose a formidable technical challenge. We present here a method to tackle this problem and analyze the number of metal nanoparticles present in different types of cells. The method relies on high-lateral-resolution (better than 30 nm) transmission x-ray microimages with both absorption contrast and phase contrast -- including two-dimensional (2D) projection images and three-dimensional (3D) tomographic reconstructions that directly show the nanoparticles.
Results:Practical tests were successfully conducted on bare and polyethylene glycol (PEG) coated gold nanoparticles obtained by x-ray irradiation. Using two different cell lines, EMT and HeLa, we obtained the number of nanoparticle clusters uptaken by each cell and the cluster size. Furthermore, the analysis revealed interesting differences between 2D and 3D cultured cells as well as between 2D and 3D data for the same 3D specimen.
Conclusions:We demonstrated the feasibility and effectiveness of our method, proving that it is accurate enough to measure the nanoparticle uptake differences between cells as well as the sizes of the formed nanoparticle clusters. The differences between 2D and 3D cultures and 2D and 3D images stress the importance of the 3D analysis which is made possible by our approach.
URI: http://ntour.ntou.edu.tw:8080/ir/handle/987654321/47277
Appears in Collections:[光電科學研究所] 博碩士論文

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