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题名: Establishment of a Trimodality Analytical Platform for Tracing, Imaging and Quantification of Gold Nanoparticles in Animals by Radiotracer Techniques
作者: Jen-Kun Chen
Chien-Hung Chen
Fong-Sian Lin
Wei-Neng Liao
Sanching L. Liang
Min-Hua Chen
Yo-Wen Chen
Wan-Yu Lin
Ming-Hua Hsu
Mei-Ya Wang
Jinn-Jer Peir
Fong-In Chou
Chin-Ya Chen
Sih-Yu Chen
Su-Chin Huang
Mo-Hsiung Yang
Dueng-Yuan Hueng
Yeukuang Hwu
Chung-Shi Yang
贡献者: 國立臺灣海洋大學:光電科學研究所
日期: 2015-01
上传时间: 2018-07-05T07:12:36Z
出版者: Anal. Chem
摘要: Abstract: This study aims to establish a (198)Au-radiotracer technique for in vivo tracing, rapid quantification, and ex vivo visualization of PEGylated gold nanoparticles (GNPs) in animals, organs and tissue dissections. The advantages of GNPs lie in its superior optical property, biocompatibility and versatile conjugation chemistry, which are promising to develop diagnostic probes and drug delivery systems. (198)Au is used as a radiotracer because it simultaneously emits beta and gamma radiations with proper energy and half-life; therefore, (198)Au can be used for bioanalytical purposes. The (198)Au-tagged radioactive gold nanoparticles ((198)Au-GNPs) were prepared simply by irradiating the GNPs in a nuclear reactor through the (197)Au(n,γ)(198)Au reaction and subsequently the (198)Au-GNPs were subjected to surface modification with polyethylene glycol to form PEGylated (198)Au-GNPs. The (198)Au-GNPs retained physicochemical properties that were the same as those of GNP before neutron irradiation. Pharmacokinetic and biodisposition studies were performed by intravenously injecting three types of (198)Au-GNPs with or without PEGylation into mice; the γ radiation in blood specimens and dissected organs was then measured. The (198)Au-radiotracer technique enables rapid quantification freed from tedious sample preparation and shows more than 95% recovery of injected GNPs. Clinical gamma scintigraphy was proved feasible to explore spatial- and temporal-resolved biodisposition of (198)Au-GNPs in living animals. Moreover, autoradiography, which recorded beta particles from (198)Au, enabled visualizing the heterogeneous biodisposition of (198)Au-GNPs in different microenvironments and tissues. In this study, the (198)Au-radiotracer technique facilitated creating a trimodality analytical platform for tracing, quantifying and imaging GNPs in animals.
關聯: 87(1)
显示于类别:[光電科學研究所] 期刊論文


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