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Please use this identifier to cite or link to this item: http://ntour.ntou.edu.tw:8080/ir/handle/987654321/47213

Title: Construction of single fluorophore ratiometric pH sensors using dual-emission Mn(2+)-doped quantum dots
Authors: Chen P
Pratiwi FW
Hsia CH
Kuo CW
Yang SM
Hwu YK
Contributors: 國立臺灣海洋大學:光電科學研究所
Keywords: Single fluorophore
D-penicillamine
Intracellular pH
Mn(2+) quantum dots
Ratiometric sensing
Date: 2016-01
Issue Date: 2018-07-05T02:24:52Z
Publisher: Biosensors and Bioelectronics
Abstract: Abstract: We present a novel ratiometric pH sensor design using water-soluble, dual-emission, Mn(2+)-doped quantum dots (Qdots) decorated with D-penicillamine (DPA-MnQdots). In contrast to more commonly used ratiometric pH sensors that rely on the coupling of two fluorophores, our design uses only a single emitter, which simplifies ratiometric sensing and broadens the applications of the sensor. Our single-emitter DPA-MnQdots exhibit two emission bands, at 510nm (green) and 610nm (red), which are, respectively, attributable to exciton recombination and emission of the Mn(2+) dopants. The emission intensity ratio (I510/I610) of the DPA-MnQdots depends linearly on surrounding pH values within physiological conditions (from pH 4.5 to 8.5). Moreover, the biocompatible DPA-MnQdots were used for long-term monitoring of local pH values in HeLa cells.
URI: http://ntour.ntou.edu.tw:8080/ir/handle/987654321/47213
Appears in Collections:[光電科學研究所] 期刊論文

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