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Please use this identifier to cite or link to this item: http://ntour.ntou.edu.tw:8080/ir/handle/987654321/46571

Title: Induction of apoptosis by Meretrix lusoria through reactive oxygen species production, GSH depletion, and caspases activation in human leukemia cells
Authors: Min-Hsiung Pan
Yu-Ting Huang
Chi-Tang Ho
Chi-I Chang
Ping-Chi Hsu
Bonnie Sun Pan
Contributors: 國立臺灣海洋大學:食品科學系
Keywords: Bcl-XL
Meretrix lusoria
Hard clam
Reactive oxygen species
Apoptosis
Cytochrome c
Caspase-9
Caspase-3
Poly(ADP-ribose) polymerase
DNA fragmentation factor
Caspase activated deoxyribonuclease
Bax
Mcl-1
Date: 2006
Issue Date: 2018-05-23T08:24:38Z
Publisher: Life Sciences
Abstract: Abstract: Apoptosis-induced directed fractionation and purification was used to identify the bioactive components of hard clams (HC), Meretrix lusoria. Two stereoisomers of epidioxysterol were previously identified as the active compounds in the ethyl acetate fraction (HC-EA). The molecular mechanism of HC-EA-induced apoptosis was also investigated in this study. Dissipation of mitochondrial membrane potential, release of mitochondrial cytochrome c into cytosol, and subsequent induction of pro-caspase-9 and -3 processing preceded apoptosis in HL-60 cells, confirmed by DNA fragmentation, chromatin condensation, changes in the cell membrane and the appearance of a sub-G1 DNA peak. Furthermore, treatment with HC-EA caused a rapid loss of intracellular glutathione content and stimulation of reactive oxygen species (ROS). Antioxidants such as catalase, N-acetylcysteine, pyrrolidine dithiocarbamate, and superoxide dismutase, but not allopurinol and diphenylene iodonium, significantly inhibited HC-EA-induced cell death. Apoptosis was completely prevented by a pan-caspase inhibitor, z-Val-Ala-Asp-fluoromethyl ketone (z-VAD-FMK). The induction of apoptosis by M. lusoria may prove to be a pivotal mechanism for its cancer chemopreventive action.
Relation: 79(12) pp.1140-1152
URI: http://ntour.ntou.edu.tw:8080/ir/handle/987654321/46571
Appears in Collections:[食品科學系] 期刊論文

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