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Please use this identifier to cite or link to this item: http://ntour.ntou.edu.tw:8080/ir/handle/987654321/46560

Title: Detection of copper ions in liquid foods and beverages based on an enzymatic method
Authors: JING-IONG YANG
DONG-BOR YEH
JEN-MIN KUO
BONNIE SUN PAN
GUO-CHI LEE
YA-HUI LIU
YING-JANG LAI
Contributors: 國立臺灣海洋大學:食品科學系
Keywords: superoxide dismutase
copper ion
apoenzyme
ELSIA microplate reader
enzymatic method
Date: 2012
Issue Date: 2018-05-23T06:57:43Z
Publisher: Journal of Food & Drug Analysis.
Abstract: Abstract: An economic, simple and sensitive enzymatic method for the determination of copper in aqueous samples was proposed in this study. By removal of copper, Superoxide dismutase (SOD) becomes apoenzyme SOD (apo-SOD) that has no SOD activity. The amount of copper added can be estimated by the recovery of SOD activity from reconstituted apo-SOD. The concentration of copper in an aqueous sample can thus be determined. A linear relationship (R2 = 0.998) between the concentrations of copper, ranging from 20 to 80 ppb, and the recovered SOD activities was observed. The detection limit for copper in the proposed assay was 20 ppb. Moreover, interfering effects of some common metals on the recovery of SOD activity was evaluated. It showed that the concentrations of these metals less than 45 µg/mL exhibited no interference in the assay. In addition, data obtained from this assay were very close to those estimated by inductively-coupled plasma atomic emission spectrometry (ICP-AES). This enzymatic method can be adapted as a routine assay for the determination of copper in aqueous samples such as beverages, drinking water and liquid foods. The assay can be applied for large number of samples (about 96 samples per hour) on an ELISA microplate reader.
Relation: 20(1) pp.83-87
URI: http://ntour.ntou.edu.tw:8080/ir/handle/987654321/46560
Appears in Collections:[食品科學系] 期刊論文

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