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Please use this identifier to cite or link to this item: http://ntour.ntou.edu.tw:8080/ir/handle/987654321/46554

Title: A Continuous Method for Measuring Calpain Activity☆
Authors: Shann-Tzong Jianga;Jeng-HwanWang;Teng;Chang;Ching-San,Chenb
Contributors: 國立臺灣海洋大學:食品科學系
Date: 1997
Issue Date: 2018-05-23T06:05:47Z
Publisher: Analytical Biochemistry
Abstract: Abstract: A simplified methodology for assaying the caseinolysis by calpains was developed. This methodology, including the incubation of calpain with casein and direct measurement of the absorbance at 500 nm, is based on the turbidity of the reaction mixture caused by the aggregation of hydrolysates during the reaction. Unlike the typical caseinolysis assay, this novel assay does not need to separate the substrate from hydrolysates and can be continuously monitored in visible wavelength range. The activity of calpain is expressed by the maximum reaction velocity (ΔA500/min) at 25°C.
Relation: 244(2) pp.233-238
URI: http://ntour.ntou.edu.tw:8080/ir/handle/987654321/46554
Appears in Collections:[食品科學系] 期刊論文

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