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Please use this identifier to cite or link to this item: http://ntour.ntou.edu.tw:8080/ir/handle/987654321/46552

Title: Technical Approach to Simplify the Purification Method and Characterization of Microbial Transglutaminase Produced from Streptoverticillium ladakanum
Authors: M.‐L. Ho
S.‐Z. Leu
J.‐F. Hsieh
S.‐T. Jiang
Contributors: 國立臺灣海洋大學:食品科學系
Keywords: properties of MTGase
purification method
Date: 2000
Issue Date: 2018-05-23T05:43:14Z
Publisher: Journal of Food Science
Abstract: In order to fast and economically purify MTGase from Streptoverticillium ladakanum, a stepwise elution method was developed and compared with linear gradient elution method. MTGase was purified to electrophoretical homogeneity by using CM Sepharose CL‐6B and Blue Sepharose Fast Flow chromatographies by linear gradient or stepwise methods. The recovery of MTGase by linear gradient and stepwise methods were 68.4% and 81.0%, respectively. The optimal temperature and pH were 40 °C and 5.5, respectively. It was stable at pH 5.0 to 7.0 and had a rate constant (KD) of 6.21 °o 10‐5 min‐1 for thermal inactivation at 45 °C. The purified MTGase was activated by K+ Na+, Ca2+, Mn2+, and Mg2+, not affected by Fe3+, EDTA, but inhibited by Cu2+, Zn2+, Hg2+, Ni2+, Co2+, Cd2+, PCMB, NEM, IAA, and PMSF. A simple stepwise method was developed for the purification of MTGase from S. ladakanum.
Relation: 65(1) pp.76-80
URI: http://ntour.ntou.edu.tw:8080/ir/handle/987654321/46552
Appears in Collections:[食品科學系] 期刊論文

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