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Title: Overexpression of the Soluble Form of Chicken Cystatin in Escherichia coli and Its Purification
Authors: Gen-Hung Chen
Shye-Jye Tang
Ching-San Chen
Shann-Tzong Jiang
Contributors: 國立臺灣海洋大學:食品科學系
Keywords: overexpression
E. coli AD494(DE3)pLysS
Chicken cystatin
cysteine proteinase inhibitor
Date: 2000
Issue Date: 2018-05-23T03:44:32Z
Publisher: Journal of Agricultural and Food Chemistry
Abstract: A cDNA encoding chicken cystatin was cloned into the pET-23a(+) expression vector and then transformed into Escherichia coli AD494(DE3)pLysS expression host. An active soluble form of cystatin was expressed in the cytoplasm of E. coli induced by isopropyl β-d-thiogalactopyranoside. The recombinant chicken cystatin was purified to electrophoretic homogeneity by a simple and rapid method involving heat treatment and Sephacryl S-100 gel filtration chromatography. The recombinant cystatin behaved as a thermal-stable protein and exhibited papain-like protease inhibition activity comparable to the natural chicken cystatin.
Relation: 48(6) pp.2602–2607
URI: http://ntour.ntou.edu.tw:8080/ir/handle/987654321/46548
Appears in Collections:[食品科學系] 期刊論文

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