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Title: Glycosylation Modification Improved the Characteristics of Recombinant Chicken Cystatin and Its Application on Mackerel Surimi
Authors: Shinn-Shuenn Tzeng
Shann-Tzong Jiang
Contributors: 國立臺灣海洋大學:食品科學系
Keywords: surimi
chicken cystatin
Pichia pastoris X-33
glycosylation modification
Date: 2004
Issue Date: 2018-05-22T08:20:49Z
Publisher: Journal of Agricultural and Food Chemistry
Abstract: Abstract: The recombinant and glycosylation chicken cystatins were expressed and secreted in the broth of Pichia pastoris X-33 transformant with apparent molecular masses (M) of 14 and 55 kDa, respectively. The glycosylation cystatin (glycocystatin) contained a polysaccharide chain that was composed of 50 DP of mannose residues. Because of the polymannosyl chain, the inhibitory ability in glycocystatin was 90.8% of recombinant cystatin. In addition to freeze−thawing stability, the thermal and pH stabilities as well as the susceptibility of glycocystatin were also enhanced. Both cystatins could improve the mackerel surimi gel by inhibiting the gel softening, which was derived from the hydrolysis of catheptic cysteine proteinases. Despite the additional amount of glycocystatin (8 units), twice that of recombinant cystatin, the 40 and 15% increases in breaking force and deformation of gels were also observed. Accordingly, the surimi gel was further improved by enhancing the stability of chicken cystatin.
Relation: 52(11) pp.3612–3616
URI: http://ntour.ntou.edu.tw:8080/ir/handle/987654321/46532
Appears in Collections:[食品科學系] 期刊論文

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