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Please use this identifier to cite or link to this item: http://ntour.ntou.edu.tw:8080/ir/handle/987654321/46521

Title: Expression of recombinant Antibacterial Lactoferricin-related peptides from Pichia pastoris Expression System
Authors: Gen-Hung Chen
Wei-Ming Chen
Guo-Ting Huang
Yu-Wen Chen
Shann-Tzong Jiang
Contributors: 國立臺灣海洋大學:食品科學系
Keywords: Pichia pastoris
Goat lactoferricin
antimicrobial peptides
Date: 2009
Issue Date: 2018-05-22T06:55:22Z
Publisher: Journal of Agricultural and Food Chemistry
Abstract: Abstract: Four recombinant antimicrobial peptide (rAMP) cDNAs, constructed from two goat lactoferricin-related peptide cDNAs (GLFcin and GLFcin II) with/without (His)6-Tag, were cloned into pPICZαC and transformed into Pichia pastoris SMD1168H. After methanol induction, these rAMPs were expressed and secreted into broth. They were purified after CM-Sepharose (without His-tg), HisTrap (with His-tg) and Sephadex G-25 chromatographies. The yield of purified rAMP was 0.15 mg/mL of broth. These 4 rAMPs were thermal-stable and with high antibacterial activity against Escherichia coli BCRC 11549, Pseudomonas aeruginosa BCRC 12450, Bacillus cereus BCRC 10603, Staphylococcus aureus BCRC 25923, Propioni bacterium acnes BCRC 10723, and Listera monocytogenes BCRC 14845. The minimum inhibitory concentration (MIC) of rAMPs against these indicators ranged from 4.07 to 16.00 mg/mL.
Relation: 57(20) pp.9509–9515
URI: http://ntour.ntou.edu.tw:8080/ir/handle/987654321/46521
Appears in Collections:[食品科學系] 期刊論文

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