English  |  正體中文  |  简体中文  |  Items with full text/Total items : 26992/38793
Visitors : 2385886      Online Users : 24
RC Version 4.0 © Powered By DSPACE, MIT. Enhanced by NTU Library IR team.
Scope Adv. Search
LoginUploadHelpAboutAdminister

Please use this identifier to cite or link to this item: http://ntour.ntou.edu.tw:8080/ir/handle/987654321/46519

Title: Bioproperties of Potent Nattokinase from Bacillus subtilis YJ1
Authors: Li-Jung Yin
Hsin-Hung Lin
Shann-Tzong Jiang
Contributors: 國立臺灣海洋大學:食品科學系
Keywords: nattokinase bioproperties and purification
Bacilus subtilis
Date: 2010
Issue Date: 2018-05-22T06:46:49Z
Publisher: Journal of Agricultural and Food Chemistry
Abstract: Abstract: Fibrinolytic enzyme activity was observed during cultivation of Bacillus subtilis YJ1 in a medium containing 1% skim milk, 1% rice husk, 0.5% NaCl, and 0.25% glucose. It was purified to electrophoretical homogeneity after CM-sepharose FF chromatography. The specific activity and yield were 1791.9 FU/mg and 9.5%, respectively. This purified fibrinolytic enzyme had M of 27.5 kDa, optimal temperature and pH at 50 °C and 8.5, respectively. It was stable at pH 6.0−10.0 and 10−40 °C and inhibited by Fe3+, Hg2+, Cu2+, Zn2+, and PMSF. Compared the N terminal of amino acids and full DNA sequence with those in NCBI, it was considered to be a nattokinase.
Relation: 58(9) pp.5737–5742
URI: http://ntour.ntou.edu.tw:8080/ir/handle/987654321/46519
Appears in Collections:[食品科學系] 期刊論文

Files in This Item:

File Description SizeFormat
index.html0KbHTML44View/Open


All items in NTOUR are protected by copyright, with all rights reserved.

 


著作權政策宣告: 本網站之內容為國立臺灣海洋大學所收錄之機構典藏,無償提供學術研究與公眾教育等公益性使用,請合理使用本網站之內容,以尊重著作權人之權益。
網站維護: 海大圖資處 圖書系統組
DSpace Software Copyright © 2002-2004  MIT &  Hewlett-Packard  /   Enhanced by   NTU Library IR team Copyright ©   - Feedback