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Title: Purification and Characterization of Acidic Protease from Aspergillus oryzae BCRC 30118
Authors: ORYZAE BCRC;Li-Jung Yin;Ya-Hui Chou;S T Jiang
Contributors: 國立臺灣海洋大學:食品科學系
Date: 2013
Issue Date: 2018-05-21T08:38:16Z
Publisher: Journal of Marine Science Technalogy
Abstract: Abstract: The acidic protease was purified from 4-day cultivation of Aspergillus oryzae BCRC 30118 by DEAE Sephacel and Sephacryl S-200 HR chromatographs. The specific activity, yield and purification fold were 117.62 kU/mg, 15.1% and 6.6, respectively. The molecular weight (M) was 41.0 kDa, while the optimal pH and temperature were 3.0 and 60°C, respectively. It was stable at pH 3.0-6.0 and 4-35°C. However, it was inhibited by Fe, Hg, Fe and pepstatin A, and slightly by leupeptin and TPCK. According to substrate specificity and inhibitor study, it was a cysteine protease with activation energy of 37.5 kcal/mol. Its Km, Vmax, Kcat and Kcat/Km for the hydrolysis of hemoglobin were 0.12 mM, 14.29 μmol/min, 14.55 sec and 125.80 (sec mM), respectively.
URI: http://ntour.ntou.edu.tw:8080/ir/handle/987654321/46513
Appears in Collections:[食品科學系] 期刊論文

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