English  |  正體中文  |  简体中文  |  Items with full text/Total items : 27533/39387
Visitors : 2541190      Online Users : 34
RC Version 4.0 © Powered By DSPACE, MIT. Enhanced by NTU Library IR team.
Scope Adv. Search
LoginUploadHelpAboutAdminister

Please use this identifier to cite or link to this item: http://ntour.ntou.edu.tw:8080/ir/handle/987654321/46513

Title: Purification and Characterization of Acidic Protease from Aspergillus oryzae BCRC 30118
Authors: ORYZAE BCRC;Li-Jung Yin;Ya-Hui Chou;S T Jiang
Contributors: 國立臺灣海洋大學:食品科學系
Date: 2013
Issue Date: 2018-05-21T08:38:16Z
Publisher: Journal of Marine Science Technalogy
Abstract: Abstract: The acidic protease was purified from 4-day cultivation of Aspergillus oryzae BCRC 30118 by DEAE Sephacel and Sephacryl S-200 HR chromatographs. The specific activity, yield and purification fold were 117.62 kU/mg, 15.1% and 6.6, respectively. The molecular weight (M) was 41.0 kDa, while the optimal pH and temperature were 3.0 and 60°C, respectively. It was stable at pH 3.0-6.0 and 4-35°C. However, it was inhibited by Fe, Hg, Fe and pepstatin A, and slightly by leupeptin and TPCK. According to substrate specificity and inhibitor study, it was a cysteine protease with activation energy of 37.5 kcal/mol. Its Km, Vmax, Kcat and Kcat/Km for the hydrolysis of hemoglobin were 0.12 mM, 14.29 μmol/min, 14.55 sec and 125.80 (sec mM), respectively.
URI: http://ntour.ntou.edu.tw:8080/ir/handle/987654321/46513
Appears in Collections:[食品科學系] 期刊論文

Files in This Item:

File Description SizeFormat
index.html0KbHTML29View/Open


All items in NTOUR are protected by copyright, with all rights reserved.

 


著作權政策宣告: 本網站之內容為國立臺灣海洋大學所收錄之機構典藏,無償提供學術研究與公眾教育等公益性使用,請合理使用本網站之內容,以尊重著作權人之權益。
網站維護: 海大圖資處 圖書系統組
DSpace Software Copyright © 2002-2004  MIT &  Hewlett-Packard  /   Enhanced by   NTU Library IR team Copyright ©   - Feedback