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Title: Liquid chromatography-tandem mass spectrometry determination of the toxicity and identification of fish species in a suspected tetrodotoxin (TTX) fish poisoning
Authors: Wu YJ;Cheng YJ;Jen HC;Pan CH;Lin TC;Lin SJ;Hwang DF.
Contributors: 國立臺灣海洋大學:食品科學系
Date: 2011
Issue Date: 2018-05-15T08:55:32Z
Publisher: Journal of Food Protection
Abstract: Abstract: Suspected tetrodotoxin (TTX) poisoning was associated with eating unknown fish in April 2009 in Taiwan. After ingestion of the fish, symptoms of the victim included perioral paresthesia, nausea, vomiting, ataxia, weakness of all limbs, respiration failure, and death within several hours. The toxicity in the remaining fish was determined, with the mice exhibiting symptoms of neurotoxin poisoning. The implicated fish and deceased victim tissues were analyzed for TTX by liquid chromatography-tandem mass spectrometry. The urine, bile, cerebrospinal fluid (spinal cord), pleural effusion, and pericardial effusion of the victim contained TTX. In addition, the partial cytochrome b gene of the implicated fish was determined by PCR. The DNA sequence in the partial 465-bp cytochrome b gene identified the implicated fish as Chelonodon patoca (puffer fish). These results indicate that people should avoid eating unknown fish species from fish markets where harvested fish may include toxic species.
Relation: 74(5):789-795
URI: http://ntour.ntou.edu.tw:8080/ir/handle/987654321/46326
Appears in Collections:[食品科學系] 期刊論文

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