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Please use this identifier to cite or link to this item: http://ntour.ntou.edu.tw:8080/ir/handle/987654321/46322

Title: Application of LC-MS/MS in identification of toxin in the causative gastropod and victim samples
Authors: Chun-lan Lin
Cheng-Hong Hsieh
You-Liang Hsieh
Yi-cheng Tai
D F Hwang
Contributors: 國立臺灣海洋大學:食品科學系
Keywords: Cooking (activity)
Gastropoda
Tetrodotoxin
Teletext
Tandem Mass Spectrometry
Adverse reaction to drug
Poisoning
Biological Assay
Purification of quantum state
Date: 2013
Issue Date: 2018-05-15T08:25:41Z
Publisher: Journal of Marine Science and Technology
Abstract: Abstract: Food paralytic poisoning incident was detected in the gastropod Nassariidae Niotha clathrata and Zeuxis scalaris occurred in December 2012 in Kaohsiung, Taiwan. The toxin was purified from each remain of cooked gastropod and victim tissues by ultrafiltration using a YM-3 membrane, followed by C18 cartridge column purification. Based on analyses by LC-MS/MS, the causative agent of gastropod food poisoning was identified as tetrodotoxin. The toxicity of remain of cooked sample was 245 MU/g (1MU = 0.178 μg TTX) for N. clathrata and 203 MU/g for Z. scalaris. The blood and excrements of victim was shown to contain TTX of 0.13 and 0.18 μg/g, respectively. There was positive between results from LC-MS/MS and mouse bioassay in the causative gastropod. The LC-MS/MS method is suitable for rapid screening of TTX.
URI: http://ntour.ntou.edu.tw:8080/ir/handle/987654321/46322
Appears in Collections:[食品科學系] 期刊論文

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