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Please use this identifier to cite or link to this item: http://ntour.ntou.edu.tw:8080/ir/handle/987654321/46205

Title: First study on gene expression of cement proteins and potential adhesion-related genes of a membranous- based barnacle as revealed from Next-Generation Sequencing technology
Authors: Hsiu-Chin Lin
Yue Him Wong
Ling Ming Tsang
Ka Hou Chu
Pei-Yuan Qian
Benny K. K. Chan
Contributors: 國立臺灣海洋大學:海洋生物研究所
Keywords: Gene Expression
Tissue membrane
Thoracica
gene function
Date: 2014
Issue Date: 2018-05-07T08:18:51Z
Publisher: Biofouling
Abstract: Abstract
This is the first study applying Next-Generation Sequencing (NGS) technology to survey the kinds, expression location, and pattern of adhesion-related genes in a membranous-based barnacle. A total of 77,528,326 and 59,244,468 raw sequence reads of total RNA were generated from the prosoma and the basis of Tetraclita japonica formosana, respectively. In addition, 55,441 and 67,774 genes were further assembled and analyzed. The combined sequence data from both body parts generates a total of 79,833 genes of which 47.7% were shared. Homologues of barnacle cement proteins - CP-19K, -52K, and -100K - were found and all were dominantly expressed at the basis where the cement gland complex is located. This is the main area where transcripts of cement proteins and other potential adhesion-related genes were detected. The absence of another common barnacle cement protein, CP-20K, in the adult transcriptome suggested a possible life-stage restricted gene function and/or a different mechanism in adhesion between membranous-based and calcareous-based barnacles.
URI: http://ntour.ntou.edu.tw:8080/ir/handle/987654321/46205
Appears in Collections:[海洋生物研究所] 期刊論文

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