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Please use this identifier to cite or link to this item: http://ntour.ntou.edu.tw:8080/ir/handle/987654321/46129

Title: Transcriptome derived microsatellite markers of Tetraclita kuroshioensis and cross amplification among Tetraclita spp.
Authors: Hsi-Nien Chen
Benny K.K.Chan
Ling Ming Tsang
Contributors: 國立臺灣海洋大學:海洋生物研究所
Keywords: Cross amplification
Date: 2015
Issue Date: 2018-05-02T08:06:51Z
Publisher: Biochemical Systematics and Ecology
Abstract: Abstract: Tetraclita barnacles are common intertidal organisms distributed in tropical and subtropical regions. These barnacles are good models for testing population dynamics and phylogeographic hypotheses in the intertidal zone. In this study, eight microsatellite markers were isolated from Tetraclita kuroshioensis through transcriptome sequencing. A total of three to eight alleles were revealed from each locus. The expected and observed heterozygosities ranged from 0.433 to 0.933 and from 0.505 to 0.762, respectively. Cross amplification among five Tetraclita barnacle species found in the Indo-West Pacific was successful with all eight microsatellite markers, although success rate varied among species. Therefore, the microsatellite markers developed in this study can be useful tools in future genetic studies with tetraclitid barnacles.
Relation: 63 pp.13-16
URI: http://ntour.ntou.edu.tw:8080/ir/handle/987654321/46129
Appears in Collections:[海洋生物研究所] 期刊論文

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