National Taiwan Ocean University Institutional Repository:Item 987654321/46102
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题名: Ethanol production efficiency of an anaerobic hemicellulolytic thermophilic bacterium, strain NTOU1, isolated from a marine shallow hydrothermal vent in Taiwan.
作者: Tsai-Ling Tsai
Shiu-Mei Liu
Shi-Chiang Lee
Wei-Jei Chen
Sheng-Hsin Chou
Tseng-Chieh Hsu
Gia-Luen Guo
Wen-Song Hwang
Juergen Wiegel
贡献者: 國立臺灣海洋大學:海洋生物研究所
关键词: rice straw
ethanol yield
Thermoanaerobacterium
hemicelluose hydrolysate
thermophile
日期: 2011
上传时间: 2018-05-02T02:50:27Z
出版者: Microbes and Environments
摘要: Abstract: A new extremely thermophilic, anaerobic, gram-negative bacterium, strain NTOU1, was enriched and isolated from acidic marine hydrothermal fluids off Gueishandao island in Taiwan with 0.5% starch and 0.5% maltose as carbon sources. This strain was capable of growth utilizing various sugars found in lignocellulosic biomass as well as xylan and cellulose, and produced ethanol, lactate, acetate, and CO2 as fermentation products. The results of a 16S rRNA gene sequence analysis (1,520 bp) revealed NTOU1 to belong to the genus Thermoanaerobacterium. When tested for the ability to grow and produce ethanol from xylose or rice straw hemicellulosic hydrolysate at 70°C, the strain showed the highest levels of ethanol production (1.65 mol ethanol mol xylose-1) in a medium containing 0.5% xylose plus 0.5% yeast extract. Maximum ethanol production from the rice straw hemicellulose was 0.509 g g-1, equivalent to 98.8% theoretical conversion efficiency. Low concentrations of inhibitors (derived from dilute acid hydrolysis) in the rice straw hemicellulose hydrolysate did not affect the ethanol yield. Thus, Thermoanaerobacterium strain NTOU1 has the potential to be used for ethanol production from hemicellulose.
關聯: 26(4) pp.317-324
URI: http://ntour.ntou.edu.tw:8080/ir/handle/987654321/46102
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