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Please use this identifier to cite or link to this item: http://ntour.ntou.edu.tw:8080/ir/handle/987654321/46100

Title: Application parameters of laccase–mediator systems for treatment of sulfonamide antibiotics
Authors: Shin-Sian Weng
Shiu-Mei Liu
Hong-Thih Lai
Contributors: 國立臺灣海洋大學:海洋生物研究所
Keywords: Toxicity
pH
Temperature
Mediator concentration
Dialysis
Date: 2013
Issue Date: 2018-05-02T02:39:12Z
Publisher: Bioresource Technology
Abstract: Abstract: This is the first study to examine the application parameters for oxidation of two sulfonamide antibiotics (SAs), sulfadimethoxine (SDM) and sulfamonomethoxine (SMM), using a novel laccase and mediators. The optimal conditions in the laccase–mediator system (LMS) were pH 4, 50–60 °C, and 1 mM for 2,2′-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) (ABTS); pH 4, 40–60 °C, and 1 mM for violuric acid (VLA); pH 6, 50 °C, and 2 mM for syringaldehyde (SIR). Additionally, the conditions of the 4-hydroxybenzyl alcohol (HBA) mediator were pH 4, 30 °C, and 2 mM in the oxidation of SDM; and the temperature increased to 60 °C for SMM. The laccase coupled with VLA and HBA resulted in the lowest toxicity of the SA solutions during processing, whereas treatments with ABTS and SIR resulted in higher toxicities. Furthermore, the laccase used in this study was stable and resistant to dialysis, thus can be reused for oxidation process.
Relation: 141 pp.152-159
URI: http://ntour.ntou.edu.tw:8080/ir/handle/987654321/46100
Appears in Collections:[海洋生物研究所] 期刊論文

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