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Please use this identifier to cite or link to this item: http://ntour.ntou.edu.tw:8080/ir/handle/987654321/45984

Title: GnRH mRNA levels in male three-spined sticklebacks, Gasterosteus aculeatus, under different reproductive conditions.
Authors: Yi Ta Shao
Yung Che Tseng
Chia-Hao Chang
Hong Young Yan
Pung Pung Hwang
Bertil Borg
Contributors: 國立臺灣海洋大學:海洋生物研究所
Keywords: mRNA
Photoperiod
Gonadectomy
GnRH2
GnRH3
Stickleback
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Date: 2015
Issue Date: 2018-04-19T01:31:14Z
Publisher: Comparative Biochemistry and Physiology Part A: Molecular & Integrative Physiology
Abstract: Abstract: In vertebrates, reproduction is regulated by the brain–pituitary–gonad (BPG) axis, where the gonadotropin-releasing hormone (GnRH) is one of the key components. However, very little is known about the possible role of GnRH in the environmental and feedback control of fish reproduction. To investigate this, full-length gnrh2 (chicken GnRH II) and gnrh3 (salmon GnRH) sequences of male three-spined sticklebacks (Gasterosteus aculeatus), which are clustered with the taxa of the same GnRH type as other Euteleostei, were cloned and annotated. gnrh1 is absent in this species. The mRNA levels of gnrh2 and gnrh3 in the sticklebacks' brain were measured under breeding and post-breeding conditions as well as in castrated and sham-operated breeding fish and castrated/sham-operated fish kept under long-day (LD 16:8) and short-day (LD 8:16) conditions. Fully breeding males had considerably higher mRNA levels of gnrh2 and gnrh3 in the thalamus (Th) and in the telencephalon and preoptic area (T + POA), respectively, than post-breeding males. Sham-operated breeding males have higher gnrh3 mRNA levels than the corresponding castrated males. Moreover, higher gnrh2 mRNA levels in the Th and higher gnrh3 mRNA levels in the T + POA and hypothalamus (HypTh) were also found in long-day sham-operated males than in sham-operated fish kept under an inhibitory short day photoperiod. Nevertheless, gnrh2 and gnrh3 mRNA levels were not up-regulated in castrated males kept under long-day photoperiod, which suggests that positive feedbacks on the brain-pituitary-gonad axis are necessary for this response.
Relation: 180 pp.6-17
URI: http://ntour.ntou.edu.tw:8080/ir/handle/987654321/45984
Appears in Collections:[海洋生物研究所] 期刊論文

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