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Please use this identifier to cite or link to this item: http://ntour.ntou.edu.tw:8080/ir/handle/987654321/45978

Title: Hormonal changes over the spawning cycle in the female three-spined stickleback, Gasterosteus aculeatus.
Authors: Chrysoula Roufidou
Monika Schmitz
Ian Mayer
Marion Sebire
Ioanna Katsiadaki
Yi Ta Shao
Bertil Borg
Contributors: 國立臺灣海洋大學:海洋生物研究所
Keywords: Teleost
Gonadotropins
Sex steroids
GnRH
Spawning cycle
Stickleback
Date: 2018
Issue Date: 2018-04-18T08:18:47Z
Publisher: General and Comparative Endocrinology
Abstract: Abstract: Female three-spined sticklebacks are batch spawners laying eggs in a nest built by the male. We sampled female sticklebacks at different time points, when they were ready to spawn and 6, 24, 48 and 72 h post-spawning (hps) with a male. Following spawning, almost all females (15 out of 19) had ovulated eggs again at Day 3 post-spawning (72 hps). At sampling, plasma, brain and pituitaries were collected, and the ovary and liver were weighed. Testosterone (T) and estradiol (E2) were measured by radioimmunoassay. Moreover, the mRNA levels of follicle-stimulating hormone (fsh-β) and luteinizing hormone (lh-β) in the pituitary, and of the gonadotropin-releasing hormones (GnRHs: gnrh2, gnrh3) and kisspeptin (kiss2) and its G protein-coupled receptor (gpr54) in the brain were measured by real-time qPCR.

Ovarian weights peaked in “ready to spawn” females, dropped after spawning, before again progressively increasing from 6 to 72 hps. Plasma T levels showed peaks at 24 and 48 hps and decreased at 72 hps, while E2 levels increased already at 6 hps and remained at high levels up to 48 hps. There was a strong positive correlation between T and E2 levels over the spawning cycle. Pituitary lh-β mRNA levels showed a peak at 48 hps, while fsh-β did not change. The neuropeptides and gpr54 did not show any changes. The changes in T and E2 over the stickleback spawning cycle were largely consistent with those found in other multiple-spawning fishes whereas the marked correlation between T and E2 does not support T having other major roles over the cycle than being a precursor for E2.
Relation: 257(1) pp.97-105
URI: http://ntour.ntou.edu.tw:8080/ir/handle/987654321/45978
Appears in Collections:[海洋生物研究所] 期刊論文

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