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Please use this identifier to cite or link to this item: http://ntour.ntou.edu.tw:8080/ir/handle/987654321/45977

Title: Genomic and proteomic analysis of structural proteins of shrimp white spot syndrome virus.
Authors: Jyh-Ming Tsai;Han-Ching Wang;Jiann-Horng Leu;He-Hsuan Hsiao;Andrew H.-J. Wang;Guang-Hsiung Kou;Chu-Fang Lo
Contributors: 國立臺灣海洋大學:海洋生物研究所
Date: 2004
Issue Date: 2018-04-18T07:55:56Z
Publisher: Journal of Virology
Abstract: Abstract: White spot syndrome virus (WSSV) virions were purified from the hemolymph of experimentally infected crayfish Procambarus clarkii, and their proteins were separated by 8 to 18% gradient sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) to give a protein profile. The visible bands were then excised from the gel, and following trypsin digestion of the reduced and alkylated WSSV proteins in the bands, the peptide sequence of each fragment was determined by liquid chromatography-nano-electrospray ionization tandem mass spectrometry (LC-nanoESI-MS/MS) using a quadrupole/time-of-flight mass spectrometer. Comparison of the resulting peptide sequence data against the nonredundant database at the National Center for Biotechnology Information identified 33 WSSV structural genes, 20 of which are reported here for the first time. Since there were six other known WSSV structural proteins that could not be identified from the SDS-PAGE bands, there must therefore be a total of at least 39 (33 + 6) WSSV structural protein genes. Only 61.5% of the WSSV structural genes have a polyadenylation signal, and preliminary analysis by 3′ rapid amplification of cDNA ends suggested that some structural protein genes produced mRNA without a poly(A) tail. Microarray analysis showed that gene expression started at 2, 6, 8, 12, 18, 24, and 36 hpi for 7, 1, 4, 12, 9, 5, and 1 of the genes, respectively. Based on similarities in their time course expression patterns, a clustering algorithm was used to group the WSSV structural genes into four clusters. Genes that putatively had common or similar roles in the viral infection cycle tended to appear in the same cluster.
Relation: 78(20) pp11360-11370
URI: http://ntour.ntou.edu.tw:8080/ir/handle/987654321/45977
Appears in Collections:[海洋生物研究所] 期刊論文

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