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Please use this identifier to cite or link to this item: http://ntour.ntou.edu.tw:8080/ir/handle/987654321/45973

Title: WSSV infection activates STAT in shrimp
Authors: Wei Yu Chen
Kun Chin Ho
Jiann Horng Leu
Kuan Fu Liu
Han Ching Wang
Guang Hsiung Kou
Chu Fang Lo
Contributors: 國立臺灣海洋大學:海洋生物研究所
Keywords: LPS
White spot syndrome virus
JAK/STAT pathway
Tyrosine phosphorylation
Date: 2008
Issue Date: 2018-04-18T07:29:10Z
Publisher: Developmental & Comparative Immunology
Abstract: Summary: Although the JAK/STAT signaling pathway is usually involved in antiviral defense, a recent study suggested that STAT might be annexed by WSSV (white spot syndrome virus) to enhance the expression of a viral immediate early gene in infected shrimps. In the present study, we clone and report the first full-length cDNA sequence for a crustacean STAT from Penaeus monodon. Alignment and comparison with the deduced amino acid sequences of other STATs identified several important conserved residues and functional domains, including the DNA binding domain, SH2 domain and C-terminal transactivation domain. Based on these conserved sequences, a phylogenetic analysis suggested that shrimp STAT belongs to the ancient STAT family, while the presence of the functional domains suggested that shrimp STAT might share similar functions and regulating mechanisms with the well-known STATs isolated from model organisms. Real-time PCR showed a decreased transcription level of shrimp STAT after WSSV infection, but a Western blot analysis using anti-phosphorylated STAT antibody showed an increased level of phosphorylated (activated) STAT in the lymphoid organ of shrimp after WSSV infection. We further show that a primary culture of lymphoid organ cells from WSSV-infected shrimp resulted in activated STAT being translocated from the cytoplasm to the nucleus. This report provides experimental evidence that shrimp STAT is activated in response to WSSV infection. Our results support an earlier finding that WSSV does not disrupt JAK/STAT pathway, but on the contrary benefits from STAT activation in the shrimp host.
Relation: 32(10) pp.1142-1150
URI: http://ntour.ntou.edu.tw:8080/ir/handle/987654321/45973
Appears in Collections:[海洋生物研究所] 期刊論文

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