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Please use this identifier to cite or link to this item: http://ntour.ntou.edu.tw:8080/ir/handle/987654321/45972

Title: Penaeus monodon caspase is targeted by a white spot syndrome virus anti-apoptosis protein.
Authors: Jiann-Horng Leu;Hao-Ching Wang;Guang-Hsiung Kou;Chu Fang Lo
Contributors: 國立臺灣海洋大學:海洋生物研究所
Date: 2008
Issue Date: 2018-04-18T07:22:35Z
Publisher: Developmental & Comparative Immunology
Abstract: Abstract: Caspases play a central and evolutionarily conserved role in mediating and executing apoptosis. Here, we report the cloning and characterization of a caspase from Penaeus monodon, Pm caspase. The full-length Pm caspase cDNA is 1386bp, encoding a polypeptide of 304 amino acids with a calculated molecular mass of 34.3kDa. BLASTP analysis against the NCBI nr database showed that Pm caspase is similar to insect effector caspases. RT-PCR analysis showed that Pm caspase mRNA is expressed in all examined tissues. When Pm caspase was overexpressed in SF-9 cells, the cells showed apoptotic morphological features, including the formation of apoptotic bodies and DNA ladders. The caspase-3 activity of Pm caspase was determined using the recombinant protein purified from Escherichia coli. Both RT-PCR and qRT-PCR analyses showed that the RNA levels of Pm caspase and P. monodon inhibitor of apoptosis protein (PmIAP) remained unchanged after white spot syndrome virus (WSSV) infection. We also used Pm caspase to show that WSSV449, an anti-apoptosis protein encoded by WSSV, is a direct caspase inhibitor.
Relation: 32(5) pp.476-486
URI: http://ntour.ntou.edu.tw:8080/ir/handle/987654321/45972
Appears in Collections:[海洋生物研究所] 期刊論文

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