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Please use this identifier to cite or link to this item: http://ntour.ntou.edu.tw:8080/ir/handle/987654321/45971

Title: Transactivation, dimerization, and DNA-binding activity of white spot syndrome virus immediate-early protein IE1
Authors: Wang-Jing Liu;Yun-Shiang Chang;Hao-Ching Wang;Jiann-Horng Leu;Guang-Hsiung Kou;Chu-Fang Lo
Contributors: 國立臺灣海洋大學:海洋生物研究所
Date: 2008
Issue Date: 2018-04-18T07:13:49Z
Publisher: Journal of Virology
Abstract: Abstract: Immediate-early proteins from many viruses function as transcriptional regulators and exhibit transactivation activity, DNA binding activity, and dimerization. In this study, we investigated these characteristics in white spot syndrome virus (WSSV) immediate-early protein 1 (IE1) and attempted to map the corresponding functional domains. Transactivation was investigated by transiently expressing a protein consisting of the DNA binding domain of the yeast transactivator GAL4 fused to full-length IE1. This GAL4-IE1 fusion protein successfully activated the Autographa californica multicapsid nucleopolyhedrovirus p35 basal promoter when five copies of the GAL4 DNA binding site were inserted upstream of the TATA box. A deletion series of GAL4-IE1 fusion proteins suggested that the transactivation domain of WSSV IE1 was carried within its first 80 amino acids. A point mutation assay further showed that all 12 of the acidic residues in this highly acidic domain were important for IE1's transactivation activity. DNA binding activity was confirmed by an electrophoresis mobility shift assay using a probe with 32P-labeled random oligonucleotides. The DNA binding region of WSSV IE1 was located in its C-terminal end (amino acids 81 to 224), but mutation of a putative zinc finger motif in this C-terminal region suggested that this motif was not directly involved in the DNA binding activity. A homotypic interaction between IE1 molecules was demonstrated by glutathione S-transferase pull-down assay and a coimmunoprecipitation analysis. A glutaraldehyde cross-linking experiment and gel filtration analysis showed that this self-interaction led to the formation of stable IE1 dimers.
Relation: 82(22) pp.11362-11373
URI: http://ntour.ntou.edu.tw:8080/ir/handle/987654321/45971
Appears in Collections:[海洋生物研究所] 期刊論文

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