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Please use this identifier to cite or link to this item: http://ntour.ntou.edu.tw:8080/ir/handle/987654321/45970

Title: White spot syndrome virus protein ICP11: A histone-binding DNA mimic that disrupts nucleosome assembly
Authors: Hao-Ching Wang;Han-Ching Wang;Tzu-Ping Ko;Yu-May Lee;Jiann-Horng Leu;Chun-Han Ho;Wei-Pang Huang;Chu-Fang Lo;Andrew H.-J. Wang
Contributors: 國立臺灣海洋大學:海洋生物研究所
Date: 2008
Issue Date: 2018-04-18T07:07:17Z
Publisher: Proc Natl Acad Sci U S A.
Abstract: Abstract: White spot syndrome virus (WSSV) is a large (≈300 kbp), double-stranded DNA eukaryotic virus that has caused serious disease in crustaceans worldwide. ICP11 is the most highly expressed WSSV nonstructural gene/protein, which strongly suggests its importance in WSSV infection; but until now, its function has remained obscure. We show here that ICP11 acts as a DNA mimic. In crystal, ICP11 formed a polymer of dimers with 2 rows of negatively charged spots that approximated the duplex arrangement of the phosphate groups in DNA. Functionally, ICP11 prevented DNA from binding to histone proteins H2A, H2B, H3, and H2A.x, and in hemocytes from WSSV-infected shrimp, ICP11 colocalized with histone H3 and activated-H2A.x. These observations together suggest that ICP11 might interfere with nucleosome assembly and prevent H2A.x from fulfilling its critical function of repairing DNA double strand breaks. Therefore, ICP11 possesses a functionality that is unique among the handful of presently known DNA mimic proteins.
Relation: 105(52) pp.20758-20763
URI: http://ntour.ntou.edu.tw:8080/ir/handle/987654321/45970
Appears in Collections:[海洋生物研究所] 期刊論文

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