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题名: Penaeus monodon thioredoxin restores the DNA binding activity of oxidized white spot syndrome virus IE1
作者: Jiun-Yan Huang;Wang-Jing Liu;Han-Ching Wang;Der-Yen Lee;Jiann-Horng Leu;Hao-Ching Wang;Mong-Hsun Tsai;Shih-Ting Kang;I-Tung Chen;Guang-Hsiung Kou;Geen-Dong Chang;Chu-Fang Lo
贡献者: 國立臺灣海洋大學:海洋生物研究所
日期: 2012
上传时间: 2018-04-18T05:41:33Z
出版者: Antioxidants & Redox Signaling
摘要: Abstract: Aims: In this study we identified viral gene targets of the important redox regulator thioredoxin (Trx), and explored in depth how Trx interacts with the immediate early gene #1 (IE1) of the white spot syndrome virus (WSSV). Results: In a pull-down assay, we found that recombinant Trx bound to IE1 under oxidizing conditions, and a coimmunoprecipitation assay showed that Trx bound to WSSV IE1 when the transfected cells were subjected to oxidative stress. A pull-down assay with Trx mutants showed that no IE1 binding occurred when cysteine 62 was replaced by serine. Electrophoretic mobility shift assay (EMSA) showed that the DNA binding activity of WSSV IE1 was downregulated under oxidative conditions, and that Penaeus monodon Trx (PmTrx) restored the DNA binding activity of the inactivated, oxidized WSSV IE1. Another EMSA experiment showed that IE1's Cys-X-X-Cys motif and cysteine residue 55 were necessary for DNA binding. Measurement of the ratio of reduced glutathione to oxidized glutathione (GSH/GSSG) in WSSV-infected shrimp showed that oxidative stress was significantly increased at 48 h postinfection. The biological significance of Trx was also demonstrated in a double-strand RNA Trx knockdown experiment where suppression of shrimp Trx led to significant decreases in mortality and viral copy numbers. Innovation and Conclusion: WSSV's pathogenicity is enhanced by the virus' use of host Trx to rescue the DNA binding activity of WSSV IE1 under oxidizing conditions. Antioxid. Redox Signal. 17, 914–926.
關聯: 17(6)
显示于类别:[海洋生物研究所] 期刊論文


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