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Please use this identifier to cite or link to this item: http://ntour.ntou.edu.tw:8080/ir/handle/987654321/45961

Title: The genome and occlusion bodies of marine Penaeus monodon nudivirus (PmNV, also known as MBV and PemoNPV) suggest that it should be assigned to a new nudivirus genus that is distinct from the terrestrial nudiviruses
Authors: Yi-Ting Yang
Der-Yen Lee
Yongjie Wang
Jer-Ming Hu
Wen-Hsiung Li
Jiann-Horng Leu
Geen-Dong Chang
Huei-Mien Ke
Shin-Ting Kang
Shih-Shun Lin
Guang-Hsiung Kou
Chu-Fang Lo
Contributors: 國立臺灣海洋大學:海洋生物研究所
Keywords: Polyhedrin
Genome
PmNV
Baculovirus
Nudivirus
OBs
Date: 2014
Issue Date: 2018-04-18T03:22:41Z
Publisher: BMC Genomics
Abstract: Abstract:Background
Penaeus monodon nudivirus (PmNV) is the causative agent of spherical baculovirosis in shrimp (Penaeus monodon). This disease causes significant mortalities at the larval stage and early postlarval (PL) stage and may suppress growth and reduce survival and production in aquaculture. The nomenclature and classification status of PmNV has been changed several times due to morphological observation and phylogenetic analysis of its partial genome sequence. In this study, we therefore completed the genome sequence and constructed phylogenetic trees to clarify PmNV’s taxonomic position. To better understand the characteristics of the occlusion bodies formed by this marine occluded virus, we also compared the chemical properties of the polyhedrin produced by PmNV and the baculovirus AcMNPV (Autographa californica nucleopolyhedrovirus).

Results
We used next generation sequencing and traditional PCR methods to obtain the complete PmNV genome sequence of 119,638 bp encoding 115 putative ORFs. Phylogenetic tree analysis showed that several PmNV genes and sequences clustered with the non-occluded nudiviruses and not with the baculoviruses. We also investigated the characteristics of PmNV polyhedrin, which is a functionally important protein and the major component of the viral OBs (occlusion bodies). We found that both recombinant PmNV polyhedrin and wild-type PmNV OBs were sensitive to acid conditions, but unlike the baculoviral OBs, they were not susceptible to alkali treatment.

Conclusions
From the viral genome features and phylogenetic analysis we conclude that PmNV is not a baculovirus, and that it should be assigned to the proposed Nudiviridae family with the other nudiviruses, but into a distinct new genus (Gammanudivirus).
Relation: 15(628)
URI: http://ntour.ntou.edu.tw:8080/ir/handle/987654321/45961
Appears in Collections:[海洋生物研究所] 期刊論文

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