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Title: The promoter of the white spot syndrome virus immediate-early gene WSSV108 is activated by the cellular KLF transcription factor.
Authors: Wang-Jing Liu
Chu-Fang Lo
Guang-Hsiung Kou
Jiann-Horng Leu
Ying-Jang Lai
Li-Kwan Chang
Yun-Shiang Chang
Contributors: 國立臺灣海洋大學:海洋生物研究所
Keywords: Penaeus monodon
White spot syndrome virus
Krüppel-like factor
Immediate-early gene
Litopenaeus vannamei
Date: 2015
Issue Date: 2018-04-18T03:15:45Z
Publisher: Developmental & Comparative Immunology
Abstract: Abstract: A series of deletion and mutation assays of the white spot syndrome virus (WSSV) immediate-early gene WSSV108 promoter showed that a Krüppel-like factor (KLF) binding site located from −504 to −495 (relative to the transcription start site) is important for the overall level of WSSV108 promoter activity. Electrophoretic mobility shift assays further showed that overexpressed recombinant Penaeus monodon KLF (rPmKLF) formed a specific protein–DNA complex with the 32P-labeled KLF binding site of the WSSV108 promoter, and that higher levels of Litopenaeus vannamei KLF (LvKLF) were expressed in WSSV-infected shrimp. A transactivation assay indicated that the WSSV108 promoter was strongly activated by rPmKLF in a dose-dependent manner. Lastly, we found that specific silencing of LvKLF expression in vivo by dsRNA injection dramatically reduced both WSSV108 expression and WSSV replication. We conclude that shrimp KLF is important for WSSV genome replication and gene expression, and that it binds to the WSSV108 promoter to enhance the expression of this immediate-early gene.
Relation: 49(1) pp.7-18
URI: http://ntour.ntou.edu.tw:8080/ir/handle/987654321/45960
Appears in Collections:[海洋生物研究所] 期刊論文

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