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Please use this identifier to cite or link to this item: http://ntour.ntou.edu.tw:8080/ir/handle/987654321/45951

Title: Molecular approaches for assessing fungal diversity in marine substrata.
Authors: Ka-Lai Pang
Julian I. Mitchell
Contributors: 國立臺灣海洋大學:海洋生物研究所
Keywords: marine fungi
ecology
PCR primers
rRNA
Date: 2005
Issue Date: 2018-04-18T01:59:50Z
Publisher: Botanica Marina
Abstract: Abstract: A range of molecular techniques is available to assess fungal diversity in natural environments. These include denaturing gradient gel electrophoresis (DGGE), temperature gradient gel electrophoresis (TGGE), single-strand conformation polymorphism (SSCP), terminal restriction fragment length polymorphism (T-RFLP), amplified rDNA restriction analysis (ARDRA), amplified ribosomal intergenic spacer analysis (ARISA) and cloning. The techniques, polymerase chain reaction (PCR) primers for environmental work and their potential limitations are discussed in this review, together with applications for describing the diversity of filamentous marine fungi associated with their substrata. It is unlikely that a single technique can be used to assess all aspects of diversity, however the limited number of studies with a molecular approach has demonstrated that marine substrata contain a greater fungal diversity than previously recognised. These studies have also shown that it is important to combine mycological and molecular approaches to accurately assess diversity.
Relation: 48(5-6)
URI: http://ntour.ntou.edu.tw:8080/ir/handle/987654321/45951
Appears in Collections:[海洋生物研究所] 期刊論文

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