National Taiwan Ocean University Institutional Repository:Item 987654321/45615
English  |  正體中文  |  简体中文  |  Items with full text/Total items : 27228/39071
Visitors : 2413991      Online Users : 67
RC Version 4.0 © Powered By DSPACE, MIT. Enhanced by NTU Library IR team.
Scope Adv. Search
LoginUploadHelpAboutAdminister

Please use this identifier to cite or link to this item: http://ntour.ntou.edu.tw:8080/ir/handle/987654321/45615

Title: Alkaline phosphatase promoter as an efficient driving element for exogenic recombinant in the marine diatom Phaeodactylum tricornutum.
Authors: Hung-Yun Lin;Shao-Chieh Yen;Po-Chih Kuo;Chih-Yu Chung;Kuan-Lin Yeh;Ching-Huei Huang;Jeng Chang;Han-Jia Lin
Date: 2017
Issue Date: 2018-03-28T08:20:51Z
Publisher: Algal Research
Abstract: Abstract: A highly inducible promoter capable of actively expressing exogenous genes in diatoms is critical to further improving the utility of this organism as a low-cost platform for industrial applications. In this study, the functional promoter of alkaline phosphatase gene in Phaeodactylum tricornutum (PtAPase) was used to develop a novel vector. The pPhAP1-EGFP vector containing the putative promoter region of PtAPase (pPhAP1) with EGFP (enhanced green fluorescent protein) open reading frame attached to downstream side. Transgenic diatom clones containing the introduced pPhAP1-EGFP vector did not express EGFP fluorescent at high phosphate concentration, and exhibit growth curves similar to that of wild type strain. EGFP fluorescent was induced and actively expressed when ambient phosphate concentration was decreased to 3.6 μM or lower. The endogenous alkaline phosphatase activity had the same expression pattern, indicating that the pPhAP1 possessed uncompromised regulatory capability. In terms of the quantity of EGFP generated by expression vectors, the yield of pPhAP1-EGFP was 9.3 to 10.5 times greater than those of other diatom vectors. These results suggested that pPhAP1 is an outstanding expression vector for diatoms with desirable characteristics of no interference with algal growth, easily tunable conditions for gene induction, and high yield of recombinant proteins.
Relation: 23
URI: http://ntour.ntou.edu.tw:8080/ir/handle/987654321/45615
Appears in Collections:[Institute of Marine Biology] Periodical Articles

Files in This Item:

File Description SizeFormat
index.html0KbHTML75View/Open


All items in NTOUR are protected by copyright, with all rights reserved.

 


著作權政策宣告: 本網站之內容為國立臺灣海洋大學所收錄之機構典藏,無償提供學術研究與公眾教育等公益性使用,請合理使用本網站之內容,以尊重著作權人之權益。
網站維護: 海大圖資處 圖書系統組
DSpace Software Copyright © 2002-2004  MIT &  Hewlett-Packard  /   Enhanced by   NTU Library IR team Copyright ©   - Feedback