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Please use this identifier to cite or link to this item: http://ntour.ntou.edu.tw:8080/ir/handle/987654321/45606

Title: Susceptibility of Marsupenaeus japonicus, P. monodon, and P. semisulcatus to white spot syndrome virus (WSSV).
Authors: Li-Li Chen
Hui-Lan Hsia
Hui-Chen Hsu
Chen-Fang Chang
Shao-En Peng
Chu-Fang Lo
Guang-Hsiung Kou
Contributors: 國立臺灣海洋大學:生命科學系
Keywords: WSSV infection
PCR
Susceptibility of shrimp
Date: 2004
Issue Date: 2018-03-28T05:46:38Z
Publisher: Journal of Fish Society
Abstract: Abstract: White spot syndrome virus (WSSV) has been detected in cultured and captured shrimps,
crabs, pest crabs and other arthopods. In this study, Penaeus japonicus postlarvae (PL28)
were experimentally challenged by WSSV via two transmission pathways: immersion and
feeding. By 15 days post-challenge, mortality in the immersion and feeding groups reached
88% to 94%. Challenge by immersion produced an earlier onset of mortality than challenge
by feeding. Only 1 out of 3 of the tested surviv ing shrimp from the immersion group was
one-step PCR positive, but all 3 tested shrimp were WSSV infected. For the feeding group,
1 out of 3 tested survivors was one-step PCR positive and 2 out of 3 were WSSV infected.
All the moribund specimens f or the immersion and feeding groups were one-step PCR
positive. The susceptibility of P. monodon, P. japonicus and P. semisulcatus to WSSV was
also determined by immersion challenge test. After 15 days, cumulative mortality reached
more than 80% in P. japonicus and P. monodon, but only 26%-47% in P. semisulcatus.
Almost all of the tested moribund P. japonicus and P. monodon samples were one-step
WSSV PCR positive. None of the P. semisulcatus were one-step PCR positive. We
concluded that P. semisulcatus is less susceptible than P. japonicus and P. monodon to
WSSV inf ection
Relation: 31(2)
URI: http://ntour.ntou.edu.tw:8080/ir/handle/987654321/45606
Appears in Collections:[生命科學系] 期刊論文

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