The Journal of DNA Mapping, Sequencing, and Analysis
Abstract: In this study, the complete mitogenome sequence of cyprinid fish, Taiwanese barbel steed, Hemibarbus schlegelii (Günther, 1868) (Teleostei: Cyprinidae) has been amplified and sequenced by employing long polymerase chain reaction method. The mitogenome, consisting of 16,612 bp, had the typical vertebrate mitochondrial gene arrangement, including 13 protein-coding genes, 22 transfer RNAs, 2 ribosomal RNAs genes and a non-coding control region (CR). CR of 933 bp length is located between tRNAPro and tRNAPhe. The overall base composition of Hemibarbus schlegelii is 29.9% for A, 27.2% for C, 25.9% for T and 17.0% for G, with a slight AT bias of 55.8%. The complete mitogenome may provide rather essential and important DNA molecular data for further phylogenetic analysis for not only congeneric species but also higher different taxa of Cyprinid fishes.