National Taiwan Ocean University Institutional Repository:Item 987654321/45593
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题名: Cloning and expression of a cDNA coding for catalase from zebrafish (Danio rerio)
作者: Chuian-Fu Ken
Chi-Tsai Lin
Jen-Leih Wu
Jei-Fu Shaw
贡献者: 國立臺灣海洋大學:生命科學系
关键词: pET-20b(+)
Danio rerio
Zebrafish
molecular cloning
cDNA
catalase
expression
Escherichia coli
RACE−PCR
日期: 2000
上传时间: 2018-03-28T01:23:17Z
出版者: Journal of Agricultural and Food Chemistry
摘要: Abstract: A full-length complementary DNA (cDNA) clone encoding a catalase was amplified by the rapid amplication of cDNA ends−polymerase chain reaction (RACE−PCR) technique from zebrafish (Danio rerio) mRNA. Nucleotide sequence analysis of this cDNA clone revealed that it comprised a complete open reading frame coding for 526 amino acid residues and that it had a molecular mass of 59 654 Da. The deduced amino acid sequence showed high similarity with the sequences of catalase from swine (86.9%), mouse (85.8%), rat (85%), human (83.7%), fruit fly (75.6%), nematode (71.1%), and yeast (58.6%). The amino acid residues for secondary structures are apparently conserved as they are present in other mammal species. Furthermore, the coding region of zebrafish catalase was introduced into an expression vector, pET-20b(+), and transformed into Escherichia coli expression host BL21(DE3)pLysS. A 60-kDa active catalase protein was expressed and detected by Coomassie blue staining as well as activity staining on polyacrylamide gel followed electrophoresis.
關聯: 48(6)
URI: http://ntour.ntou.edu.tw:8080/ir/handle/987654321/45593
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