National Taiwan Ocean University Institutional Repository:Item 987654321/45590
English  |  正體中文  |  简体中文  |  全文筆數/總筆數 : 28611/40652
造訪人次 : 776435      線上人數 : 62
RC Version 4.0 © Powered By DSPACE, MIT. Enhanced by NTU Library IR team.
搜尋範圍 進階搜尋


題名: Purification and characterization of the copper/zinc-superoxide dismutase from black porgy.
作者: Chi-Tsai Lin;T.-L. Lee;K.-J. Duan;J.-C. Su
貢獻者: 國立臺灣海洋大學:生命科學系
日期: 2001
上傳時間: 2018-03-27T08:18:07Z
出版者: Zoological studies
摘要: Abstract: Purification and characterization of black porgy muscle Cu/Zn superoxide dismutase. Zoological Studies 40(2): 84-90. Superoxide dismutase (SOD) has been proposed to be used as a bioindicator for environmental impact assessment. From a survey of SOD activity in black porgy, Acanthopagrus schlegeli, we found that Cu/Zn SOD was distributed rather evenly in 6 tissues, and in addition, only heart, liver, and testis had Mn SOD. We purified Cu/Zn SOD from muscle to homogeneity by a procedure that includes heating at 65 °C and fractionation on 2 chromatographic columns. The molecular mass of the native enzyme was 33 kDa and that of the subunit mass, deduced from a cDNA sequence, was 15.85 kDa. Thus the native enzyme appeared to be a homodimer. It had an N-terminal sequence of VLKAVCVLKGAGQTTGVV. The specific activity was 3318 u/mg. The enzyme had a broad optimum pH range of 5.8 to 11.2 and was resistant both to proteolysis by trypsin and chymotrypsin and to heat denaturation. The thermal inactivation rate constant of the enzyme at 80 °C was -0.0237 min-1 and the half life for inactivation was 27.8 min.
關聯: 40(2)
顯示於類別:[生命科學系] 期刊論文


檔案 描述 大小格式瀏覽次數



著作權政策宣告: 本網站之內容為國立臺灣海洋大學所收錄之機構典藏,無償提供學術研究與公眾教育等公益性使用,請合理使用本網站之內容,以尊重著作權人之權益。
網站維護: 海大圖資處 圖書系統組
DSpace Software Copyright © 2002-2004  MIT &  Hewlett-Packard  /   Enhanced by   NTU Library IR team Copyright ©   - 回饋