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Please use this identifier to cite or link to this item: http://ntour.ntou.edu.tw:8080/ir/handle/987654321/45564

Title: Identification of Histone Demethylases in Sacchromyces cerevisiae.
Authors: Shengjiang Tu;Esther M. M. Bulloch;Lanhao Yang;Chen Ren;Wei-Chieh Huang;Pang-Hung Hsu;Chein-Hung Chen;Chung-Lin Liao;Hui-Ming Yu;Wan-Sheng Lo;Michael A. Freitas;Ming-Daw Tsai
Contributors: 國立臺灣海洋大學:生命科學系
Date: 2007
Issue Date: 2018-03-26T07:28:54Z
Publisher: The Journal of Biological Chemistry
Abstract: Abstract: Based on the prediction that histone lysine demethylases may contain the JmjC domain, we examined the methylation patterns of five knock-out strains (ecm5Δ, gis1Δ, rph1Δ, jhd1Δ, and jhd2Δ (yjr119cΔ)) of Saccharomyces cerevisiae. Mass spectrometry (MS) analyses of histone H3 showed increased modifications in all mutants except ecm5Δ. High-resolution MS was used to unequivocally differentiate trimethylation from acetylation in various tryptic fragments. The relative abundance of specific fragments indicated that histones K36me3 and K4me3 accumulate in rph1Δ and jhd2Δ strains, respectively, whereas both histone K36me2 and K36me accumulate in gis1Δ and jhd1Δ strains. Analyses performed with strains overexpressing the JmjC proteins yielded changes in methylation patterns that were the reverse of those obtained in the complementary knock-out strains. In vitro enzymatic assays confirmed that the JmjC domain of Rph1 specifically demethylates K36me3 primarily and K36me2 secondarily. Overexpression of RPH1 generated a growth defect in response to UV irradiation. The demethylase activity of Rph1 is responsible for the phenotype. Collectively, in addition to Jhd1, our results identified three novel JmjC domain-containing histone demethylases and their sites of action in budding yeast S. cerevisiae. Furthermore, the methodology described here will be useful for identifying histone demethylases and their target sites in other organisms.
URI: http://ntour.ntou.edu.tw:8080/ir/handle/987654321/45564
Appears in Collections:[生命科學系] 期刊論文

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