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Please use this identifier to cite or link to this item: http://ntour.ntou.edu.tw:8080/ir/handle/987654321/45539

Title: A type III ACC synthase, ACS7, is involved in root gravitropism in Arabidopsis thaliana.
Authors: Huang SJ
Chang CL
Wang PH
Tsai MC
Hsu PH
Chang IF.
Contributors: 國立臺灣海洋大學:生命科學系
Keywords: root gravitropism.
ACS
14-3-3
calcium
CDPK
ethylene
phosphorylation
Date: 2013
Issue Date: 2018-03-26T01:29:04Z
Publisher: Journal of Experimental Botany
Abstract: Abstract: Ethylene is an important plant hormone that regulates developmental processes in plants. The ethylene biosynthesis pathway is a highly regulated process at both the transcriptional and post-translational level. The transcriptional regulation of these ethylene biosynthesis genes is well known. However, post-translational modifications of the key ethylene biosynthesis enzyme 1-aminocyclopropane-1-carboxylate (ACC) synthase (ACS) are little understood. In vitro kinase assays were conducted on the type III ACS, AtACS7, fusion protein and peptides to determine whether the AtACS7 protein can be phosphorylated by calcium-dependent protein kinase (CDPK). AtACS7 was phosphorylated at Ser216, Thr296, and Ser299 by AtCDPK16 in vitro. To investigate further the function of the ACS7 gene in Arabidopsis, an acs7-1 loss-of-function mutant was isolated. The acs7-1 mutant exhibited less sensitivity to the inhibition of root gravitropism by treatment with the calcium chelator ethylene glycol tetraacetic acid (EGTA). Seedlings were treated with gradient concentrations of ACC. The results showed that a certain concentration of ethylene enhanced the gravity response. Moreover, the acs7-1 mutant was less sensitive to inhibition of the gravity response by treatment with the auxin polar transport inhibitor 1-naphthylphthalamic acid, but exogenous ACC application recovered root gravitropism. Altogether, the results indicate that AtACS7 is involved in root gravitropism in a calcium-dependent manner in Arabidopsis.
Relation: 64(14)
URI: http://ntour.ntou.edu.tw:8080/ir/handle/987654321/45539
Appears in Collections:[生命科學系] 期刊論文

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