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Please use this identifier to cite or link to this item: http://ntour.ntou.edu.tw:8080/ir/handle/987654321/45505

Title: Pyrrolysine‐Inspired Protein Cyclization
Authors: Lee MM
Fekner T
Lu J
Heater BS
Behrman EJ
Zhang L
Hsu PH
Chan MK.
Contributors: 國立臺灣海洋大學:生命科學系
Keywords: pyrrolysine analogues
cyclic peptides
protein engineering
Date: 2014
Issue Date: 2018-03-22T03:18:34Z
Publisher: Chembiochem
Abstract: Abstract: The pyrrolysine translational machinery has been extensively explored for the production of recombinant proteins containing a variety of "site-specific" non-canonical amino acids for both in vitro and in vivo biochemical studies. In this study, we report the first use of this technology for the production of branched cyclic proteins with a tadpole-like topology. As a proof of concept, we fused the well-studied RGD peptide to the C terminus of an mCherry reporter protein. Previous studies have shown that cyclization of the RGD peptide enhances its internalization into cells compared to its linear counterpart. The cellular uptake efficiencies of mCherry-cyclo(RGD), mCherry-linear(RGD), and wild-type mCherry determined by flow cytometry follow the trends expected, thereby confirming the feasibility and potential utility of this cyclization approach.
Relation: 15(12)
URI: http://ntour.ntou.edu.tw:8080/ir/handle/987654321/45505
Appears in Collections:[生命科學系] 期刊論文

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