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Please use this identifier to cite or link to this item: http://ntour.ntou.edu.tw:8080/ir/handle/987654321/45491

Title: Detection of urinary spermine by using silver-gold/silver chloride nanozymes.
Authors: Po-Chih Kuo
Chia-Wen Lien
Ju-Y Mao
Binesh Unnikrishnan
Huan-Tsung Chang
Han-Jia Lin
Chih-Ching Huang
Contributors: 國立臺灣海洋大學:生命科學系
Keywords: anti-tumor activity
immune protection
Date: 2018
Issue Date: 2018-03-21T07:47:03Z
Publisher: Analytica Chimica Acta
Abstract: Abstract: In this paper, we report a simple one-step synthesis method for silver-gold bimetallic nanoparticles deposition on silver chloride nanosheets to form Ag-Au/AgCl nanohybrid with oxidase-like and peroxidase-like catalytic activity. We used these nanohybrid in the detection of spermine. First, 13 nm-sized Au NPs were synthesized by citrate reduction of HAuCl4 solution, and then, Ag+ ions were added to the solution without any purification. The added Ag+ reacted with the Cl− ions in the dispersion, thus immediately forming AgCl nanosheets through a precipitation reaction, and the aurophilic interactions with the Au NPs resulted in the formation and in situ self-deposition of Ag-Au NPs on the AgCl nanosheets at room temperature. We investigated the enzyme-mimicking activity of the Ag-Au/AgCl nanohybrid in detail via the O2- or H2O2-Amplex Red (AR) redox system. The Ag-Au/AgCl nanohybrid exhibited at least 150-fold higher catalytic activity than that of Ag-Au NPs or AgCl nanosheets, due to synergistic effect. Spermine inhibited the enzyme-mimic activity of the Ag-Au/AgCl nanohybrid, thereby allowing for the construction of a probe for detecting nanomolar concentrations of spermine in urine samples. This cost-effective sensing system was used to easily and rapidly detect the concentrations of spermine in complex urine samples.
Relation: 1009
URI: http://ntour.ntou.edu.tw:8080/ir/handle/987654321/45491
Appears in Collections:[生命科學系] 期刊論文

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