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Please use this identifier to cite or link to this item: http://ntour.ntou.edu.tw:8080/ir/handle/987654321/45453

Title: Flavonoids, centaurein and centaureidin, from Bidens pilosa, stimulate IFNgamma expression.
Authors: Shu-Lin Chang;Yi-Ming Chiang;Cicero Lee-Tian Chang;Hsu-Hua Yeh;Lie-Fen Shyur;Yueh-Hsiung Kuo;Tung-Kung Wu;Wen-Chin Yang
Contributors: 國立臺灣海洋大學:水產養殖學系
Date: 2007
Issue Date: 2018-03-19T07:44:02Z
Publisher: Journal of Ethnopharmacology
Abstract: Abstract: Bidens pilosa is used as an ethnical medicine for bacterial infection or immune modulation in Asia, America and Africa. Here, we employed an IFN-γ promoter-driven luciferase reporter construct and T cells to characterize immunomodulatory compounds from this plant based on a bioactivity-guided isolation principle. We found that PHA, a positive control, caused a six-fold increase in IFN-γ promoter activity. In contrast, hot water crude extracts from Bidens pilosa and its butanol subfraction increased IFN-γ promoter activity to two- and six-fold, respectively. Finally, centaurein (EC50 = 75 μg/ml) and its aglycone, centaureidin (EC50 = 0.9 μg/ml), isolated from this butanol subfraction, augmented IFN-γ promoter activity by ∼four-fold. Consistent with the role of centaurein or its aglycone in IFN-γ regulation, we showed that centaurein induced the activity of NFAT and NFκB enhancers, located within the IFN-γ promoter, in Jurkat cells. Overall, our results showed that centaurein regulated IFN-γ transcription, probably via NFAT and NFκB in T cells.
Relation: 112(2)
URI: http://ntour.ntou.edu.tw:8080/ir/handle/987654321/45453
Appears in Collections:[水產養殖學系] 期刊論文

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