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Title: Environmental and cortisol-mediated control of Ca2+ uptake in tilapia (Oreochromis mossambicus)
Authors: Chia-Hao Lin
Wei-Chun Kuan
Bo-Kai Liao
Ang-Ni Deng
Deng-Yu Tseng
Pung-Pung Hwang
Contributors: 國立臺灣海洋大學:水產養殖學系
Keywords: Tilapia
Ca2+ influx
Date: 2016
Issue Date: 2018-03-15T01:16:27Z
Publisher: Journal of Comparative
Abstract: Abstract: Ca2+ is a vital element for many physiological processes in vertebrates, including teleosts, which live in aquatic environments and acquire Ca2+ from their surroundings. Ionocytes within the adult gills or larval skin are critical sites for transcellular Ca2+ uptake in teleosts. The ionocytes of zebrafish were found to contain transcellular Ca2+ transporters, epithelial Ca2+ channel (ECaC), plasma membrane Ca2+-ATPase 2 (PMCA2), and Na+/Ca2+ exchanger 1b (NCX1b), providing information about the molecular mechanism of transcellular Ca2+ transports mediated by ionocytes in fish. However, more evidence is required to establish whether or not a similar mechanism of transcellular Ca2+ transport also exists in others teleosts. In the present study, ecac, pmca2, and ncx1 were found to be expressed in the branchial ionocytes of tilapia, thereby providing further support for the mechanism of transcellular Ca2+ transport through ionocytes previously proposed for zebrafish. In addition, we also reveal that low Ca2+ water treatment of tilapia stimulates Ca2+ uptake and expression of ecac and cyp11b (the latter encodes a cortisol-synthesis enzyme). Treatment of tilapia with exogenous cortisol (20 mg/l) enhanced both Ca2+ influx and ecac expression. Therefore, increased cyp11b expression is suggested to enhance Ca2+ uptake capacity in tilapia exposed to low Ca2+ water. Furthermore, the application of cortisol receptor antagonists revealed that cortisol may regulate Ca2+ uptake through glucocorticoid and/or mineralocorticoid receptor (GR and/or MR) in tilapia. Taken together, the data suggest that cortisol may activate GR and/or MR to execute its hypercalcemic action by stimulating ecac expression in tilapia.
Relation: 186
URI: http://ntour.ntou.edu.tw:8080/ir/handle/987654321/45363
Appears in Collections:[水產養殖學系] 期刊論文

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