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Title: Development and application of a monoclonal antibody against a putative myristoylated membrane protein encoded by grouper iridovirus 59L gene.
Authors: Zhi-Yu Chen;Pinwen Peter Chiou;Chian-Jiun Liou;Yu-Shen Lai
Contributors: 國立臺灣海洋大學:水產養殖學系
Date: 2015-04
Issue Date: 2018-03-14T07:56:10Z
Publisher: Diseases of Aquatic Organisms
Abstract: Abstract: Groupers (Epinephelus spp.) are economically important fish species worldwide, and ranaviruses are major viral pathogens causing heavy economic losses in grouper aquaculture. In this study, the 59L gene of grouper iridovirus (GIV-59L) was cloned and characterized. This gene is 1521 bp and encodes a protein of 506 amino acids with a predicted molecular mass of 53.9 kDa. Interestingly, GIV-59L and its homologs are found in all genera of the family Iridoviridae. A mouse monoclonal antibody specific for the C-terminal domain (amino acid positions 254-506) of the GIV-59L protein, GIV-59L(760-1518)-MAb-21, was produced and proved to be well suited for use in a number of GIV immunoassays. RT-PCR, Western blotting, and cycloheximide and cytosine arabinoside drug inhibition analyses indicated that GIV-59L is a viral late gene in GIV-infected grouper kidney cells. Immunofluorescence analysis revealed that GIV-59L protein mainly accumulates in the cytoplasm of infected cells and is finally packed into a whole virus particle. The GIV-59L(760-1518)-MAb-21 characterized in this study could have widespread application in GIV immunodiagnostics and other research on GIV. In addition, the results presented here offer important insights into the pathogenesis of GIV.
Relation: 113(3)
URI: http://ntour.ntou.edu.tw:8080/ir/handle/987654321/45360
Appears in Collections:[水產養殖學系] 期刊論文

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