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Please use this identifier to cite or link to this item: http://ntour.ntou.edu.tw:8080/ir/handle/987654321/45338

Title: A simple and rapid PCR-based method for ostrich sexing using micro amounts of DNA
Authors: C-W Huang;M-C Huang
Contributors: 國立臺灣海洋大學:水產養殖學系
Date: 2008-10
Issue Date: 2018-03-14T03:10:57Z
Publisher: Reproduction in Domestic Animals
Abstract: Abstract: The aim of the present study is to identify ostrich sex by using polymerase chain reaction (PCR) on micro amounts of DNA from blood, bloodstain and feathers. Sixteen male and 18 female ostriches were used as test individuals. Genomic DNA as a template was extracted by the Chelex method. Ostrsex-P1 and P2 primers were designed to perform PCR amplification on the template. PCR products were checked using agarose gel electrophoresis with ethidium bromide staining and ostrich sex was determined directly by the bands shown on the gel. The results demonstrate that ostrich sex can be determined by the extraction of DNA from as little as 0.0125 microl blood using Chelex, whereby the use of large amounts of organic solvents such as phenol and chloroform are unnecessary. In addition, it is possible to identify ostrich sex using micro amounts of DNA extracted from bloodstains and/or feathers. The use of feathers particularly avoids unwanted sampling problems such as the difficulty of collecting ostrich blood, the stress to the ostrich caused by bleeding, and the demand for a lot of manpower for ostrich restraint.
Relation: 43
URI: http://ntour.ntou.edu.tw:8080/ir/handle/987654321/45338
Appears in Collections:[水產養殖學系] 期刊論文

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