National Taiwan Ocean University Institutional Repository:Item 987654321/45337
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题名: Expressed transcripts associated with high rates of egg production in chicken ovarian follicles.
作者: K.T.Yanga;C.Y.Lin;H.L.Huang;J.S.Liou;C.Y.Chien;C.P.Wu;C.W.Huang;B.R.Ou;C.F.Chen;Y.P.Lee;E.C.Lin;P.C.Tang;W.C.Lee;S.T.Ding;W.T.K.Cheng;M.C.Huang
贡献者: 國立臺灣海洋大學:水產養殖學系
日期: 2008
上传时间: 2018-03-14T03:03:44Z
出版者: Molecular and Cellular Probes
摘要: Abstract: The purpose of this study was to characterize differentially expressed transcripts associated with varying rates of egg production in Taiwan country chickens. Ovarian follicles were isolated from two strains of chicken which showed low (B) or high (L2) rates of egg production, then processed for RNA extraction and cDNA library construction. Three thousand and eight forty clones were randomly selected from the cDNA library and amplified by PCR, then used in microarray analysis. Differentially expressed transcripts (P<0.05, log2⩾1.75) were sequenced, and aligned using GenBank. This analysis revealed 20 non-redundant sequences which corresponded to known transcripts. Eight transcripts were expressed at a higher level in ovarian tissue prepared from chicken strain B, and 12 transcripts were expressed at a higher level in L2 birds. These differential patterns of expression were confirmed by semi-quantitative RT-PCR. We show that transcripts of cyclin B2 (cycB2), ferritin heavy polypeptide 1 (FTH1), Gag-Pol polyprotein, thymosin β4 (TB4) and elongation factor 1 α1 (EEF1A1) were enriched in B strain ovarian follicles. In contrast, thioredoxin (TXN), acetyl-CoA dehydrogenase long chain (ACADL), inhibitor of growth family member 4 (ING4) and annexin II (ANXA2) were expressed in at higher levels in the L2 strain. We suggest that our approach may lead to the isolation of effective molecular markers that can be used in selection programs in Taiwan country chickens.
關聯: 22
URI: http://ntour.ntou.edu.tw:8080/ir/handle/987654321/45337
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