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Please use this identifier to cite or link to this item: http://ntour.ntou.edu.tw:8080/ir/handle/987654321/45336

Title: Differentially expressed transcripts in shell glands from low and high egg production strains of chickens using cDNA microarrays
Authors: Kuo-Tai Yang
Chia-Yu Lin
Jong-Shian Liou
Yi-Hsing Fan
Shiow-Her Chiou
Chang-Wen Huang
Chean-Ping Wua
En-Chung Lin
Chih-Feng Chen
Yen-Pai Lee
Wen-Chuan Lee
Shih-Torng Ding
Winston Teng-Kuei Cheng
Mu-Chiou Huang
Contributors: 國立臺灣海洋大學:水產養殖學系
Keywords: RT-PCR
Shell glands
Chickens
Egg productionc
DNA microarrays
Date: 2007
Issue Date: 2018-03-14T02:59:09Z
Publisher: Animal Reproduction Science
Abstract: Abstract: We have constructed a tissue-specific in-house cDNA microarray to identify differentially expressed transcripts in shell glands from low (B) and high (L2) egg production strains of Taiwanese country chickens during their egg-laying period. The shell gland cDNA library was constructed from the high egg production strain. cDNA clones (7680) were randomly selected and their 5′-end sequences characterized. After excluding overlapping sequences, an in-house cDNA microarray, representing 2743 non-redundant transcripts, was generated for functional genomic studies. Using our microarray, we have successfully identified 85 differentially expressed transcripts from the two different strains of chicken shell glands. In this study, 34 of these transcripts were associated with signal transduction, protein biosynthesis, cell adhesion, cellular metabolism, skeletal development, cell organization and biogenesis. We selected a number of the differentially expressed transcripts for further validation using semi-quantitative RT-PCR. These included elongation factor 2 (EEF2), ovocalyxin-32 (OCX-32) and annexin A2 (ANXA2) which were expressed at high levels in the chicken shell glands of the B strain and, in contrast, the coactosin-like protein (COTL1), transcription factor SOX18 and MX protein were more highly expressed in the L2 strain. Our results suggest that these differentially expressed transcripts may be suitable to use as molecular markers for high rates of egg production, and now need to be investigated further to assess whether they can be applied for use in breeding selection programs in Taiwanese country chickens.
Relation: 101
URI: http://ntour.ntou.edu.tw:8080/ir/handle/987654321/45336
Appears in Collections:[水產養殖學系] 期刊論文

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