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Please use this identifier to cite or link to this item: http://ntour.ntou.edu.tw:8080/ir/handle/987654321/45334

Title: Female-specific DNA sequences in geese.
Authors: Huang MC;Lin WC;Horng YM;Rouvier R;Huang CW.
Contributors: 國立臺灣海洋大學:水產養殖學系
Date: 2003
Issue Date: 2018-03-14T02:36:50Z
Publisher: British Poultry Science
Abstract: Abstract: The OPAE random primers (Operon Technologies, Inc., CA) were used for random amplified polymorphic DNA (RAPD) fingerprinting in Chinese, White Roman and Landaise geese. One of these primers, OPAE-06, produced a 938-bp sex-specific fragment in all females and in no males of Chinese geese only. 2. A novel female-specific DNA sequence in Chinese goose was cloned and sequenced. Two primers, CGSex-F and CGSex-R, were designed in order to amplify a 912-bp sex-specific polymerase chain reaction (PCR) fragment on genomic DNA from female geese. 3. It was shown that a simple and effective PCR-based sexing technique could be used in the three goose breeds studied. 4. Nucleotide sequencing of the sex-specific fragments in White Roman and Landaise geese was performed and sequence differences were observed among these three breeds.
Relation: 44
URI: http://ntour.ntou.edu.tw:8080/ir/handle/987654321/45334
Appears in Collections:[水產養殖學系] 期刊論文

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