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Please use this identifier to cite or link to this item: http://ntour.ntou.edu.tw:8080/ir/handle/987654321/45320

Title: Development of immersion subunit vaccine against grouper iridovirus (GIV).
Authors: Hong-Jen Liang
Ren-Hong Peng
Pinwen P Chiou
Contributors: 國立臺灣海洋大學:水產養殖學系
Keywords: subunit vaccine
major capsid protein
Date: 2016-06
Issue Date: 2018-03-13T06:41:17Z
Publisher: Fish & Shellfish Immunology
Abstract: Abstract: Iridovirus is one of the most devastating viral pathogens in groupers (Epinephelus spp). The viral
infection could often lead to near 100% mortality in hatcheries, thus a bottleneck to the development of
the grouper aquaculture industry. Vaccination is an important and efficient strategy against viral infection.
The most common delivery method for commercially available fish vaccines is injection, which is both
time-consuming and labor intensive despite the high efficacy. We report here the development of a new
adjuvant NE and its application in an immersion GIV subunit vaccine. The adjuvant NE can form low
viscosity nanoparticles with average size of about 168 nm and are safe to grouper. When co-delivered
with NE, green fluorescent protein (GFP) can be detected in gills and the digestive tract at 20 min postimmersion.
Subsequently, an immersion subunit vaccine was generated by combining NE with
recombinant major capsid protein (MCP) of GIV. After vaccination by immersion, MCP-specific
antibodies could be detected at 2 weeks post-vaccination. Furthermore, the subunit vaccine enhanced the
survival rate of infected fish in a dose-dependent manner. We further evaluated the vaccination protocol
of this subunit vaccine. The results showed that boosters (at 1 or 2 weeks after the initial vaccination)
enhanced the yield of specific antibodies and the protection against GIV in the vaccinated grouper at both
3 and 4 weeks after initial vaccination. Booster at 1 week had a greater impact than booster at 2 week did.
At 10 weeks after initial vaccination, the fish were resistant to GIV in all groups; however, the viral loads
in the booster groups was lower than those in the single-vaccination and control groups. In conclusion,
out study indicates the potential application of this immersion subunit GIV vaccine as an effective and
easy-to-handle vaccine for the grouper aquaculture industry.
Relation: 53
URI: http://ntour.ntou.edu.tw:8080/ir/handle/987654321/45320
Appears in Collections:[水產養殖學系] 期刊論文

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