National Taiwan Ocean University Institutional Repository:Item 987654321/45063
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题名: Salinity preference of silvering Japanese eel Anguilla japonica:evidence from pituitary prolactin mRNA levels and otolith Sr:Ca ratios
作者: Han, Y.S.;Yu, Y.L.;Liao, I C.;Tzeng, W.N.
日期: 2003
上传时间: 2017-12-27T02:05:10Z
出版者: Marine Ecology Progress Series
摘要: The salinity preference of Japanese eel Anguilla japonica during silvering was validated from the viewpoints of physiological ecology by examining the pituitary prolactin (PRL) mRNA expression and the otolith strontium: calcium (Sr:Ca) ratios of the eels of different sexual maturity stages collected in an estuary of Taiwan. The PRL possessed a putative signal peptide of 24 amino acids and a mature peptide of 185 amino acids. RT-PCR and real-time quantitative PCR analyses indicated that the transcript levels of PRL mRNA significantly decreased from yellow to silver stage. On the contrary, the Sr:Ca ratios at the otolith edge of silver eels, which recorded their latest experience of salinity history, significantly increased. The increase in otolith Sr:Ca ratios and the decrease in PRL mRNA expression are consistent with the timing of migration from freshwater to seawater during silvering of the eel.
關聯: 259, pp.253-261
URI: http://ntour.ntou.edu.tw:8080/ir/handle/987654321/45063
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