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|Title: ||Electroporation of sperm to introduce foreign DNA into the genome of loach (Misgurnus anguillicaudatus)|
|Authors: ||Huai-Jen Tsai;F. S. Tseng;I Ching Liao|
|Issue Date: ||2017-12-13T02:09:10Z
|Publisher: ||Canadian Journal of Fisheries and Aquatic Sciences|
|Abstract: ||An antifreeze protein gene promoter from ocean pout (Macrozoarces americanus) linked to a chinook salmon (Oncorhynchus tshawytscha) growth hormone cDNA (opAFPGHc) was introduced into loach (Misgurnus anguillicaudatus) sperm via electroporation under the following conditions: amplitude, 8 kV; burst time, 1.6 s; number of pulses; 28; pulse time, 120 μs; cycle, 6. Compared with the untreated sperm, the fertilization rate driven by the electroporated sperm was decreased slightly, with no significant effect on hatchability. The success rate of gene transfer was 50%. Genomic Southern blotting demonstrated that DNA from transgenic loach contained positive band(s) with higher (37.5%), the same, or lower molecular size as the transferred opAFPGHc fragment. There was a mosaic distribution of exogenous DNA in a wide variety of tissues analyzed. The average weight gain between the 14th and 161th day of the experimental group was 2.5-fold higher than those of the control and mock-treated groups. The body length distribution of the experimental group was rather widely scattered instead of being normally distributed.
Electroporation of sperm to introduce foreign DNA into the genome of loach... | Request PDF. Available from: https://www.researchgate.net/publication/237184612_Electroporation_of_sperm_to_introduce_foreign_DNA_into_the_genome_of_loach_Misgurnus_anguillicaudatus [accessed Dec 13 2017].
|Relation: ||52(4), pp.776-787|
|Appears in Collections:||[廖一久院士專區] 期刊論文|
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